• Home

  • About SPF Biotech

  • Products and services

  • Sales service

  • Download

  • Join us

    • Products

    Products

    Technical support and service
    Products and services
    Products
    C57BL/6JNifdc mice
    C57BL was obtained by inbreeding the offspring of female mice 57 and male mice 52 from Abby Lathrop mice bred by C.C. Little in 1921. C57BL/6 and C57BL/10 sublines were separated in 1937. In 2011, Spafas (Beijing) Biotechnology Co., Ltd. introduced and bred C57BL/6JNifdc mice from the National Rodent Laboratory Animal Resources.
    Features:

    Low spontaneous tumor incidence, strong resistance and adaptability, high reproductive and survival rates.

    Prone to diet-induced obesity, high alcohol tolerance, low levels of adrenaline-like lipids.

    High interferon production, moderate tolerance to radiation, high complement activity, relatively easy to induce immune tolerance.

    Susceptible to histamine factors of pertussis, sensitive to tuberculosis bacteria.

    Applications

    Genetics, immunology, neurobiology, and neurology research.

    Cardiovascular biology, developmental biology, models of diabetes and obesity, myocardial infarction models, renal failure models, and preparation of monoclonal and polyclonal antibodies.

    Growth curve
    Physiological data

    品系周龄

    C57BL/6J（8 weeks）

    指标

    单位

    雄

    雌

    白细胞计数

    WBC (109/L)

    4.03±0.71

    3.39±0.75

    中性粒细胞计数

    Neu (109/L)

    0.43±0.1

    0.38±0.1

    淋巴细胞计数

    Lym (109/L)

    3.56±0.66

    2.97±0.64

    单核细胞计数

    Mon (109/L)

    0.03±0.03

    0.03±0.01

    嗜酸性粒细胞计数

    Eos (109/L)

    0.01±0.01

    0.02±0.01

    嗜碱性粒细胞计数

    Bas (109/L)

    0±0

    0±0

    嗜中性粒细胞比例

    Neu (%)

    10.75±1.96

    10.9±1.73

    淋巴细胞比例

    Lym (%)

    88.25±2.35

    87.42±1.77

    单核细胞比例

    Mon (%)

    0.71±1.04

    0.94±0.28

    嗜酸性粒细胞比例

    Eos (%)

    0.18±0.25

    0.68±0.34

    嗜碱性粒细胞比例

    Bas (%)

    0.12±0.08

    0.07±0.07

    红细胞计数

    RBC (1012/L)

    8.52±0.27

    7.73±0.25

    血红蛋白浓度

    HGB (g/L)

    132.65±3.98

    122.4±3.81

    红细胞压积

    HCT (%)

    39.86±1.27

    38.14±0.87

    平均红细胞体积

    MCV (fL)

    46.84±0.68

    49.36±0.98

    平均红细胞血红蛋白含量

    MCH (pg)

    15.59±0.3

    15.82±0.24

    平均红细胞血红蛋白浓度

    MCHC (g/L)

    333.05±4.72

    320.9±5.52

    红细胞分布宽度变异系数

    RDW-CV (%)

    11.57±0.65

    12.78±1.03

    红细胞分布宽度标准差

    RDW-SD (fL)

    22.05±1.39

    27.53±2.51

    血小板计数

    PLT (109/L)

    1051.65±127.98

    760.55±175.58

    平均血小板体积

    MPV (fL)

    4.77±0.12

    4.66±0.11

    血小板体积分布宽度

    PDW

    15.02±0.12

    15.15±0.09

    血小板压积

    PCT (%)

    0.5±0.05

    0.35±0.07

    品系周龄

    C57BL/6J（8 weeks）

    指标

    单位

    雌

    雄

    谷丙转氨酶

    ALT

    U/L

    27.36±3.16

    19.86±2.81

    谷草转氨酶

    AST

    U/L

    119.55±27.59

    73.6±22.63

    血清甘油三酯

    TG

    mmol/L

    1.95±0.59

    1.14±0.33

    胆固醇

    TC

    mmol/L

    1.94±0.27

    2.15±0.26

    血糖

    Glu-G

    mmol/L

    3.06±0.89

    4.26±0.91

    碱性磷酸酶

    ALP

    U/L

    168.52±27.79

    146.7±33.11

    γ-谷氨酰转肽酶

    γ-GT

    U/L

    1.02±0.31

    0.64±0.28

    血清总蛋白

    TP

    g/L

    50.32±12.9

    42.51±4.72

    α-淀粉酶

    α-AMY

    U/L

    2159.94±343.77

    1885.17±262.85

    肌酸激酶

    CK

    U/L

    939.21±316.47

    792.51±411.14

    乳酸脱氢酶

    LDH

    U/L

    248.6±69.71

    227.53±44.57

    血清白蛋白

    ALB Ⅱ

    g/L

    30.06±1.48

    26.34±3.53

    脂肪酶

    LIP

    U/L

    27.45±6.73

    25.3±5.44

    尿素氮

    UREA

    mmol/L

    9.03±1.04

    8.51±1.03

    血清钙

    Ca

    mmol/L

    22.19±0.07

    1.77±0.14

    血清磷

    P

    mmol/L

    2.38±0.13

    1.89±0.21

    血清镁

    Mg Ⅱ

    mmol/L

    1.47±6.1

    1.36±0.13
    样本信息和目的
    • 样本：
      C57BL/6J Nifdc 小鼠
    • 来源：
      斯贝福
    • 目的：
      通过SNP位点检测确认C57BL/6J小鼠遗传类型
    • 方法：
      DNA提取、PCR扩增和Sanger测序
    C57BL/6J Nifdc小鼠SNP位点基因型信息

    Locus
    No.

    dbSNP ID

    染色体

    Position (bp)
    (GRCm38)

    C57BL/6J allele

    C57BL/6NJ allele

    8车间6J

    1

    rs246236360

    1

    11996705

    C/C

    T/T

    C/C

    2

    rs212521754

    1

    16,968,405

    C/C

    A/A

    C/C

    3

    rs213024334

    1

    30167141

    C/C

    T/T

    C/C

    4

    rs244794780

    1

    40107883

    T/T

    A/A

    T/T

    5

    rs260670033

    1

    50146448

    C/C

    T/T

    C/C

    6

    rs249907793

    1

    61950812

    C/C

    A/A

    C/C

    7

    rs265151779

    1

    83182637

    G/G

    A/A

    G/G

    8

    rs229124202

    1

    89861338

    G/G

    T/T

    G/G

    9

    rs223540754

    1

    110024886

    G/G

    C/C

    G/G

    10

    rs259683638

    1

    119116297

    C/C

    T/T

    C/C

    11

    rs229911289

    1

    132980179

    T/T

    A/A

    T/T

    12

    rs262282675

    1

    188434376

    A/A

    T/T

    A/A

    13

    rs251979693

    2

    11214185

    C/C

    T/T

    C/C

    14

    rs228546410

    2

    41205764

    T/T

    A/A

    T/T

    15

    rs254996546

    2

    51969852

    C/C

    T/T

    C/C

    16

    rs256541267

    2

    70251451

    C/C

    T/T

    C/C

    17

    rs214356625

    2

    96674180

    G/G

    T/T

    G/G

    18

    rs224344563

    2

    102710505

    A/A

    T/T

    A/A

    19

    rs253212197

    2

    164813748

    C/C

    T/T

    C/C

    20

    rs213376233

    2

    170240435

    G/G

    A/A

    G/G

    21

    rs221521392

    2

    181,868,891

    C/C

    T/T

    C/C

    22

    rs222821429

    3

    66305330

    C/C

    T/T

    C/C

    23

    rs262827930

    3

    109597274

    A/A

    T/T

    A/A

    24

    rs254145219

    3

    147657255

    C/C

    T/T

    C/C

    25

    rs219227155

    4

    19328298

    T/T

    C/C

    T/T

    26

    rs235104023

    4

    56463984

    C/C

    T/T

    C/C

    Locus
    No.

    dbSNP ID

    染色体

    Position (bp)
    (GRCm38)

    C57BL/6J allele

    C57BL/6NJ allele

    8车间6J

    27

    rs256724446

    5

    35,701,259

    G/G

    A/A

    G/G

    28

    rs260260338

    5

    80026465

    G/G

    A/A

    G/G

    29

    rs221990668

    5

    150224989

    G/G

    T/T

    G/G

    30

    rs257294810

    6

    39971164

    G/G

    A/A

    G/G

    31

    rs224103578

    7

    53390545

    C/C

    T/T

    C/C

    32

    rs229340185

    7

    140821590

    A/A

    T/T

    A/A

    33

    rs255341040

    8

    58790625

    G/G

    A/A

    G/G

    34

    rs239219835

    8

    79117401

    G/G

    A/A

    G/G

    35

    rs214490504

    9

    60662109

    G/G

    A/A

    G/G

    36

    rs51123066

    10

    11070460

    G/G

    T/T

    G/G

    37

    rs213583872

    10

    49357252

    G/G

    T/T

    G/G

    38

    rs246274290

    10

    88,091,833

    T/T

    C/C

    T/T

    39

    rs223857079

    11

    12253003

    A/A

    T/T

    A/A

    40

    rs240617401

    11

    46222615

    G/G

    A/A

    G/G

    41

    rs231656457

    12

    29886947

    T/T

    G/G

    T/T

    42

    rs217422777

    12

    70772479

    C/C

    T/T

    C/C

    43

    rs226310424

    13

    41494375

    G/G

    C/C

    G/G

    44

    rs251507217

    13

    101,112,155

    A/A

    T/T

    A/A

    45

    rs242991609

    13

    119477808

    C/C

    A/A

    C/C

    46

    rs235428682

    14

    75727727

    G/G

    A/A

    G/G

    47

    rs222607275

    14

    117850332

    G/G

    A/A

    G/G

    48

    rs243245803

    15

    22748238

    T/T

    G/G

    T/T

    49

    rs243400512

    15

    55816925

    C/C

    T/T

    C/C

    50

    rs231321125

    15

    97760563

    C/C

    G/G

    C/C

    51

    rs230243864

    16

    20458800

    C/C

    T/T

    C/C

    52

    rs240948896

    16

    61450798

    G/G

    A/A

    G/G

    53

    rs240067957

    17

    40854409

    C/C

    T/T

    C/C

    54

    rs259144033

    17

    69131609

    C/C

    T/T

    C/C

    55

    rs255789242

    18

    59,519,801

    C/C

    T/T

    C/C

    56

    rs263687961

    18

    90448757

    A/A

    G/G

    A/A

    57

    rs232414357

    19

    23329888

    C/C

    T/T

    C/C

    58

    rs230656170

    19

    40364531

    G/G

    A/A

    G/G

    59

    rs266019057

    X

    112095948

    A/A

    G/G

    A/A

    60

    rs212226666

    X

    157445480

    T/T

    A/A

    T/T
     
    结果分析

    以上 60 个 SNP 位点信息与文献中报道的 C57BL/6J 小鼠基因类型 100% 一致，且各位点未存在杂合子现象。

    结论

    斯贝福（北京）生物技术有限公司 C57BL/6J 小鼠为近交系 C57BL/6J 小鼠。

    HOT RECOMMEND